常规三代全长引物列表
| 测序类型 | 引物名称 | 引物序列 | 区域 | 文献 | 备注 |
细菌16S rRNA | 27F | 5’-AGAGTTTGATCMTGGCTCAG-3’ | 16s V1-V9 全长1.5k | [1] | Pacbio 平台 |
1492R | 5’- CRGYTACCTTGTTACGACTT-3’ | ||||
| 真菌ITS | ITS1 | 5'-CTTGGTCATTTAGAGGAAGTAA-3’ | ITS 全长 700bp | [2] | Pacbio 平台 |
ITS4 | 5'-TCCT CCGC TTAT TGAT ATGC-3’ | ||||
| 偏好产甲烷古菌 | A1F | 5′-GKTTGATCCYGSCRGAG-3′ | 16S全长1.5k | [3] | Pacbio 平台 |
1490R | 5′-GGYTACCTTGTTACGACTT-3’ |
常规引物列表
| 测序类型 | 引物名称 | 引物序列 | 区域 | 文献 | 备注 |
| 细菌16S rRNA | 341F | 5’-CCTAYGGGRBGCASCAG-3’ | 16s V3-V4 | [4] | PE250平台 |
| 806R | 5’-GGACTACNNGGGTATCTAAT-3’ | ||||
| 细菌16S rRNA | 515F | 5’- GTGCCAGCMGCCGCGG-3’ | 16s V4-V5 | [5] | PE250平台 |
| 907R | 5’- CCGTCAATTCMTTTRAGTTT-3’ | ||||
| 细菌16S rRNA | 515F | 5’- GTGCCAGCMGCCGCGG-3’ | 16s V4 | [6] | PE250平台 |
| 806R | 5’- GGACTACHVGGGTWTCTAAT-3’ | ||||
| 细菌16S rRNA | 799F | 5’- AACMGGATTAGATACCCKG-3’ | 16s V5-V6 | [7] | PE250平台 |
| 1115R | 5’- AGGGTTGCGCTCGTTG-3’ | ||||
真菌ITS | ITS1F | 5’- CTTGGTCATTTAGAGGAAGTAA-3’ | ITS1 | [8] | PE250平台 |
| ITS2R | 5’- GCTGCGTTCTTCATCGATGC-3’ | ||||
| 真核18S | TAReuk454FWD1 | 5’-CCAGCASCYGCGGTAATTCC-3’ | 18s V4 | [9] | PE250平台 |
| TAReukREV3 | 5’-ACTTTCGTTCTTGATYRA-3’ | ||||
| 古菌 | Arch519F | 5’- CAGCCGCCGCGGTAA-3’ | V4-V5 | [10] | PE250平台 |
Arch915R | 5’- GTGCTCCCCCGCCAATTCCT-3’ | ||||
| 鱼类（海水） | MiFish_U-F | 5’-GTTGGTAAATCTCGTGCCAGC-3’ | eDNA 12S rRNA | [11] | PE150平台 |
MiFish_U-R | 5’CATAGTGGGGTATCTAATCCTAGTTTG-3’ | ||||
| 鱼类（淡水） | Tele02_F | 5′-AAACTCGTGCCAGCCACC-3′ | eDNA 12S rRNA | [12] | PE150平台 |
Tele02_R | 5′-GGGTATCTAATCCCAGTTTG-3′ | ||||
| 真核COI | MlCOIintF | 5’GGWACWGGWTGAACWGTWTAYC CYCC-3’ | eDNA | [13] | PE250平台 |
JghHCO2198 | 5’-TAIACYTCIGGRTGICCRAARAAYCA-3’ | ||||
| 产甲烷菌 | Met86F | 5’- GCTCAGTAACACGTGG-3’ | 产甲烷菌 | [14] | Pacbio 平台 |
Met1340R | 5’-CGGTGTGTGCAAGGAG-3’ | ||||
| 固氮菌 | NifH1F | 5’- TGYGAYCCNAARGCNGA -3’ | 固氮细菌 | [15] | PE250平台 |
NifH2R | 5’- ADNGCCATCATYTCNCC-3’ | ||||
| 丛枝真菌 | AMV4.5NF | 5′- AAGCTCGTAGTTGAATTTCG-3’ | SSU 300bp | [16] | PE250平台 |
AMDGR | 5′-CCCAACTATCCCTATTAATCAT-3’ |
非常规引物列表
| 测序类型 | 引物名称 | 引物序列 | 区域 | 文献 | 备注 |
| 真核18S | 1380F | 5’-CCCTGCCHTTTGTACACAC -3’ | 18s V9 | [17] | PE250平台 |
| 1510R | 5’-CCTTCYGCAGGTTCACCTAC-3’ | ||||
| 真核18S | 0817F | 5’-TTAGCATGGAATAATRRAATAGGA-3' | 18S V5-V7 | [18] | PE250平台 |
| 1196R | 5'-TCTGGACCTGGTGAGTTTCC-3' | ||||
| 真核18S | SSU_FO4 | 5’-GCTTGTCTCAAAGATTAAGCC-3’ | 18S V1-V2 | [19] | PE250平台 |
| SSU_R22 | 5’-GCCTGCTGCCTTCCTTGGA-3’ | ||||
| 古菌 | ArchU519F | 5’-CAGYMGCCRCGGKAAHACC-3’ | Arch 16s V4 | [20] | PE250平台 |
| Arch806R | 5’-GGACTACNSGGGTMTCTAAT-3’ | ||||
氨氧化细菌 | amoA-1F | 5'-GGGGTTTCTACTGGTGGT-3' | 氨氧化细菌 | [21] | PE250平台单端分析 |
| a moA-2R | 5'-CCCCTCKGSAAAGCCTTCTTC-3' | ||||
氨氧化古菌 | Arch-amoAF | 5’-STAATGGTCTGGCTTAGACG-3’ | 氨氧化古菌 | [22] | PE250平台单端分析 |
| Arch-amoAR | 5’-GCGGCCATCCATCTGTATGT-3’ | ||||
线虫 | NF1 | 5’-GCTGGTGCATGGCCCTTCTTACTT-3' | 线虫18S | [23] | PE250平台 |
| 18Sr2bR | 5'-TACAAAGCGCAGCGACCTAAT-3' | ||||
| 反硝化细菌 (nirS) | Cd3aF | 5’-GTSAACGTSAAGGARACSGG-3’ | nirS | [24] | PE250平台 |
| R3cd | 5’-GASTTCGGRTGSGTCTTGA-3’ | ||||
厌氧真菌 | MN100 | 5’-TCCTACCCTTTGTGAATTTG-3’ | 厌氧真菌 | [25] | PE250平台 |
| MNGM2C | 5’-CTGCGTTCTTCATCGTTGCG-3’ | ||||
| 产甲烷古菌 | Met86F | 5’- GCTCAGTAACACGTGG-3’ | 产甲烷古菌 | [26] | PE250平台 |
| Met471R | 5’-GWRTTACCGCGGCKGCTG-3’ | ||||
反硝化 细菌(nirK) | nirK583F | 5’- TCATGGTGCTGCCGCGKGACGG-3’ | nirK | [27] | PE250平台 |
| nirK909R | 5’-GAACTTGCCGGTKGCCCAGAC-3’ | ||||
反硝化细 菌(nosZ) | nosZ-F | 5’-CGYTGTTCMTCGACAGCCAG-3’ | nosZ | [28] | PE250平台 |
| nosZ1622R | 5’-CGSACCTTSTTGCCSTYGCG-3’ | ||||
反硝化细 菌（cnorB) | cnorB2F | 5’- GACAAGNNNTACTGGTGGT - 3’ | cnorB | [29] | PE250平台 |
| cnorB6R | 5’- GAANCCCCANACNCCNGC-3’ | ||||
厌氧氨氧化菌 | AMX368F | 5’-TTCGCAATGCCCGAAAG-3’ | Anammox | [30] | PE250平台 |
| AMX820R | 5’-AAAACCCCTCTACTTAGTGCCC-3’ | ||||
硫酸盐还原菌 | APS1F | 5’-TGGCAGATCATGATYMAYGG-3’ | aprA | [31] | PE250平台 |
| APS4R | 5’- GCGCCAACYGGRCCRTA- 3’ | ||||
藻类 | Cyan359F | 5’-GGGGAATYTTCCGCAATGGG- 3’ | chloroplasts | [32] | PE250平台 |
| Cyan781R | 5’-GACTACWGGGGTATCTAATCCCWTT- 3’ | ||||
真核18S | 528F | 5’-GCGGTAATTCCAGCTCCAA-3’ | 18s V4 350bp | [33] | PE250平台 |
| 706R | 5’-AATCCRAGAATTTCACCTCT-3’ | ||||
大型藻类 | F | 5’-CCAGCASCYGCGGTAATTCC- 3’ | 18S V9 | [34] | PE150平台 |
| R | 5’-CCTTCYGCAGGTTCACCTA- 3’ | ||||
| 大型藻类 | Euka02-F | 5’-TTTGTCTGSTTAATTSCG- 3’ | 18S V7 | [34] | PE150平台 |
| Euka02-R | 5’-CACAGACCTGTTATTGC- 3’ |
注明：功能引物为非常规引物，需要收合成费用；
甲方可以提供特殊引物，但是需要经过乙方的确认后收费合成。
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